From 4c4cb997115a6bbd8d448ae5ee45d641f8be7ec5 Mon Sep 17 00:00:00 2001 From: ocrampal Date: Fri, 1 May 2026 12:38:34 +0200 Subject: [PATCH] messo a posto i titoli e iniziato il tun vgcc --- neuron/presynapse.md | 147 +++++++++++++++++++++++-------------------- 1 file changed, 79 insertions(+), 68 deletions(-) diff --git a/neuron/presynapse.md b/neuron/presynapse.md index c3c2e42..ef1fc0f 100644 --- a/neuron/presynapse.md +++ b/neuron/presynapse.md @@ -133,10 +133,7 @@ container: PRESYNAPSE ### AP_ctx: PRESYNAPSE -#### ms: AP_ctx: PRESYNAPSE - - -##### NTreleaseLow: interacting +#### NTreleaseLow: int: ms Il rilascio di NT lo facciamo nel contesto di AP. Biologicamente dovrebbe avvenire solo in base alle concentrazioni, quindi anche al difuori degli AP. @@ -168,7 +165,7 @@ interacting: NTreleaseLow trace: None ``` -##### NTreleaseMedium: interacting +#### NTreleaseMedium: int: ms In tutti i casi di NT @@ -186,7 +183,7 @@ interacting: NTreleaseMedium trace: None ``` -##### NTreleaseHigh: interacting +#### NTreleaseHigh: int: ms ```Gen interacting: NTreleaseHigh @@ -201,14 +198,17 @@ interacting: NTreleaseHigh trace: None ``` -##### Ca2+TracesAccumulationLow: interacting +#### Ca2+TracesAccumulationLow: int: ms -Serve a dare la velocita' al trasporto di vesicles da RP a RRP. - - The biological meaning is that a synapse that has just been through a burst is primed for fast recovery — the molecular machinery for vesicle docking is already engaged, calcium-dependent priming factors are still elevated, and the system is in a ready state. A synapse that has been silent for several seconds has cooled down and replenishes slowly. +Serve a: +- fare il tuning dei VGCC +- dare la velocita' al trasporto di vesicles da RP a RRP, anche se non avendo ancora compreso ATP, la velocita' non cambia molto la sostanza. +- le tracce vengono eliminate quando il neurone e' in pausa, lontano da uno spike train, TunPossible_ctx +- Abbiamo 3 tracce, high, medium and low. Andiamo a verificare una combinazione di queste per fare la modulazione +- RF e' a 10, questo dovrebbe essere un RF di campionamento durante AP_ctx context che dovremmo assicurarci sia tipo 100. Il che implicherebbe 10 campionamenti. +- The biological meaning is that a synapse that has just been through a burst is primed for fast recovery — the molecular machinery for vesicle docking is already engaged, calcium-dependent priming factors are still elevated, and the system is in a ready state. A synapse that has been silent for several seconds has cooled down and replenishes slowly. +- So after one second of silence CaTrace has fallen to ~37% of its peak value, after two seconds to ~14%, after three seconds to ~5%. It asymptotes toward zero but never exactly reaches it. Between spikes, Ca2+ falls toward zero as the pumps clear it. The result is that CaTrace encodes not the instantaneous calcium level but the recent history of calcium activity — a smoothed, time-averaged measure of how active the synapse has been over the past one to two seconds. - - So after one second of silence CaTrace has fallen to ~37% of its peak value, after two seconds to ~14%, after three seconds to ~5%. It asymptotes toward zero but never exactly reaches it. Between spikes, Ca2+ falls toward zero as the pumps clear it. The result is that CaTrace encodes not the instantaneous calcium level but the recent history of calcium activity — a smoothed, time-averaged measure of how active the synapse has been over the past one to two seconds. - - Abbiamo 3 tracce, high, medium and low. Andiamo a verificare se una di queste e' high per fare la modulazione - - RF e' a 10, questo dovrebbe essere un RF di campionamento durante AP_ctx context che dovremmo assicurarci sia tipo 100. Il che implicherebbe 10 campionamenti. --- ```Gen @@ -223,7 +223,7 @@ interacting: Ca2+TracesAccumulationLow trace: None ``` -##### Ca2+TracesAccumulationMedium: interacting +#### Ca2+TracesAccumulationMedium: int: ms ```Gen interacting: Ca2+TracesAccumulationMedium @@ -237,7 +237,7 @@ interacting: Ca2+TracesAccumulationMedium trace: None ``` -##### Ca2+TracesAccumulationHigh: interacting +#### Ca2+TracesAccumulationHigh: int: ms ```Gen @@ -254,11 +254,7 @@ interacting: Ca2+TracesAccumulationHigh ### NOT AP_ctx: PRESYNAPSE -#### ms: NOT AP_ctx: PRESYNAPSE - -#### sec: NOT AP_ctx: PRESYNAPSE - -##### eCB clearance: interacting +#### eCB clearance: int: ms eCB dipende da POST. Tende a modulare l'entrata di Ca2+ degli VGCC. @@ -276,33 +272,7 @@ interacting: eCBClearance trace: None ``` -##### Ca2+TracesClearance: interacting - -Qui non facciamo un flush di CaTraceX, riduciamo ogni mezzo secondo (context) di un RF di questo episodio. - -```Gen -interacting: Ca2+TracesClearance - contained_by: PRESYNAPSE - - in_context: NOT AP_ctx - rf: ( active: 30x ) # Slow - - hypothesis: NOT (CaTraceHigh empty) - action: [CaTRaceHigh decrease] - trace: None - - hypothesis: NOT (CaTraceMedium empty) - action: [CaTRaceMedium decrease] - trace: None - - hypothesis: NOT (CaTraceLow empty) - action: [CaTRaceLow decrease] hypothesis: NOT (CaTraceHigh empty) - action: [CaTRaceHigh decrease] - trace: None - trace: None -``` - -##### RPShuttleLow: interacting +#### RPShuttleLow: int: sec This happens in the seconds loop, once per second. @@ -324,7 +294,7 @@ interacting: RPShuttleLow trace: None ``` -##### RPShuttleMedium: interacting +#### RPShuttleMedium: int: sec The "Sub-Optimal" State. The machinery is working, but it's held back by partial limitations. This covers cases where the signal is steady but the "piston" isn't firing at full speed, or where a high vacancy in the RRP (emptiness) forces a low signal to work a bit harder. @@ -345,7 +315,7 @@ interacting: RPShuttleMedium trace: None ``` -##### RPShuttleHigh: interacting +#### RPShuttleHigh: int: sec The "High Performance" State. Multiple systems are optimized, but one is still at a "mediumness" level. This represents an active synapse that hasn't reached its absolute peak because either the supply is only 50% or the RRP isn't empty enough to create that "maximal vacuum" pull. @@ -365,9 +335,7 @@ interacting: RPShuttleHigh trace: None ``` -#### min: NOT AP_ctx: PRESYNAPSE - -##### RefillRPGlutamine +#### RefillRPGlutamine: int: min This happens in the minutes loop, once per minute, via the glutamine shuttle from the astrocyte. It is a two-step process across two cells. @@ -397,14 +365,40 @@ Gln — depletes over bursts, refilled by glucose (slowest, astrocyte-depend Each tier buys time for the one below it to respond. When all three are depleted simultaneously — which only happens under prolonged high-frequency firing with insufficient glucose — the synapse has no remaining buffer and goes silent until the minutes loop restores the Glutamine_pool. -### VGCC-PRE-TUN: Tuner +### TunPossible_ctx: PRESYNAPSE + +#### Ca2+TracesClearance: int: sec + +Qui facciamo un flush di CaTraceX. + +```Gen +interacting: Ca2+TracesClearance + contained_by: PRESYNAPSE + + in_context: NOT AP_ctx + rf: ( active: 30x ) # Slow + + hypothesis: NOT (CaTraceHigh empty) + action: [CaTRaceHigh decrease] + trace: None + + hypothesis: NOT (CaTraceMedium empty) + action: [CaTRaceMedium decrease] + trace: None + + hypothesis: NOT (CaTraceLow empty) + action: [CaTRaceLow decrease] + trace: None +``` + +## VGCC-PRE-TUN: Tuner ```Gen tuner: VGCC-PRE-TUN # qui stiamo aggiungendo o eliminando VGCC-PRE. Fra un massimo full e minimo empty (empty puo' non essere 0) - contained_by: PRESYNAPSE + # contained_by: PRESYNAPSE non e' contenuto, si attacca. tunes: PRESYNAPSE/expansion/VCGG-PRE @@ -419,7 +413,11 @@ tuner: VGCC-PRE-TUN tub_intricated: ``` -#### CheckVgccPreTun:contexting +### TunPossible_ctx: VGCC-PRE-TUN + +#### CheckVgccPreTun: contexting: sec + +Qui controlliamo che ci siano le condizioni per aumentare o diminuire la quantita' di VGCC ```Gen contexting: CheckVgccPreTun @@ -428,17 +426,34 @@ contexting: CheckVgccPreTun in_context: TunPossible_ctx rf: ( active: 60x ) - condition: - out_context: TryTunPreVcgg_ctx + condition: ( CaTraceHigh fullness ) + out_context: TryTunUpPreVcgg_ctx + + condition: ( CaTraceLow fullness ) + out_context: TryTunDownPreVcgg_ctx ``` -#### PossibleVgccPreTun: interacting +#### PossibleUpPreVgccTun: int: sec ```Gen -interacting: PossibleVgccPreTun +interacting: PossibleUpPreVgccTun contained_by: VGCC-PRE-TUN - in_context: TryTunPreVcgg_ctx + in_context: TryTunUpPreVcgg_ctx + rf: ( active: 10x ) + + hypothesis: + action: + trace: +``` + +#### PossibleDownPreVgccTun: int: sec + +```Gen +interacting: PossibleDownPreVgccTun + contained_by: VGCC-PRE-TUN + + in_context: TryTunDownPreVcgg_ctx rf: ( active: 10x ) hypothesis: @@ -465,9 +480,7 @@ container: VGCC-PRE Da rivedere le condizioni per aggiungere mGluR che ha come proxy NT concentration!!!!! -#### ms: AP_ctx: VGCC-PRE - -##### Ca2+enterLow: interacting +#### Ca2+enterLow: int: ms Here we comprehend the breaking activity on VGCC by: CDI, eCB and mGluR: @@ -499,7 +512,7 @@ interacting: Ca2+enterLow trace: None ``` -##### Ca2+enterMedium: interacting +#### Ca2+enterMedium: int: ms ```Gen interacting: Ca2+enterMedium @@ -515,7 +528,7 @@ interacting: Ca2+enterMedium trace: None ``` -##### Ca2+enterHigh: interacting +#### Ca2+enterHigh: int: ms ```Gen interacting: Ca2+enterHigh @@ -532,9 +545,7 @@ interacting: Ca2+enterHigh ### NOT AP_ctx: VGCC-PRE -#### ms: NOT AP_ctx: VGCC-PRE - -##### Ca2+ClearanceLow: interacting +#### Ca2+ClearanceLow: int: ms Qui eliminiamo Ca2+. Il tempo che ci mette ad eliminare il Ca2+ dovrebbe essere minoe dell'inervallo fra un AP_ctx e un'altra. Siccome non comprendiamo per ora ATP, non c'e' accumulo di Ca2+ per mancanza di ATP (stanchezza). @@ -560,7 +571,7 @@ interacting: Ca2+ClearanceLow trace: None ``` -##### Ca2+ClearanceHigh: interacting +#### Ca2+ClearanceHigh: int: ms ```Gen interacting: Ca2+ClearanceHigh