NTrelease episodes

neuron/BEH-AXO.md

@@ -93,7 +93,7 @@ container: BEH-AXO
 **Tubs:**
 
 - **Ca2+**: Calcium Ion entering the Presynapse when VCGG open. They are key to check the concentration, release vescicles and modulation
-- **CaMicro**: Free calcium Ions that influence NT release.
+- **Ca2Micro**: free cytosolic Ca2+ in microdomain that influence NT release. Normally returns to ~0 between spikes; stays elevated when pumps fail
 - **ReadyReleasablePool**: Readily Releasable Pool: The Readily Releasable Pool consists of the vesicles that are "docked" and "primed" at the active zone of the synapse.  
 -- Location: Directly touching the presynaptic membrane.  
 -- Function: These are the first to be released when an action potential arrives.  
@@ -130,10 +130,119 @@ container: BEH-PRE
     - Rp ( fullness: 30x, active: 15x, emptiness: 0x )
        # developed_by: DEV-PRE-RRP-FULL from DEV.N
 
-    - TagRelease ( fullness: 1x, active: 0x, emptiness: 0x ) 
-
     - CaTraces ( fullness: 50x, active: 0x, emptiness: 0x )
 
-  tub_intricated:   
+  tub_intricated:
+    - NT
+
+  context_intricated:
+  - AP ( contained_by: BEH-SOMA ) 
+
 ```
 
+### RRPConcentration: Context
+
+```Gen
+context: RRPConcentration
+  contained_by: BEH-PRE
+
+  in_context: AP
+  rf: ( active: 60x )
+
+  condition: NOT (Rrp empty) AND NOT (Rrp full) 
+    out_context: RRPMedium
+
+  condition: (Rrp full) 
+    out_context: RRPFull
+```
+
+### Ca2MicroConcentration: Context
+
+```Gen
+context: Ca2MicroConcentration
+  contained_by: BEH-PRE
+
+  in_context: AP
+  rf: ( active: 60x )
+
+  condition: NOT (Ca2Micro empty) AND NOT (Ca2Micro full) 
+    out_context: Ca2MicroMedium
+
+  condition: (Ca2Micro full) 
+    out_context: Ca2MicroFull
+```
+
+### NTrelease episodes
+
+Ci sono 4 casi che dipendono da RRP, CaMicro e NT. L'idea e' che la quantita' di RRP sia il driver principale. Gli NT liberati sono di piu' al crescere di RRP e CaMicro e di meno al crescere di NT. Gli NT nella sinapsi fanno da moderazione alla ulteriore liberazione di NT, ma non bloccano mai totalmente. NT suppression only matters when everything else is already at maximum — which is exactly the biological purpose: it prevents runaway release during peak activity, not during moderate activity.
+
+#### NTreleaseMaximum episodes
+
+```Gen
+episode: NTreleaseMaximum
+  contained_by: BEH-PRE
+
+  in_context: (Ca2MicroFull AND RRPFull)
+  rf: ( active: 6x ) # Maximum
+
+  hypothesis: (NT empty)
+    action: [Rrp decrease, Nt increase, ATP decrease]
+    trace: None
+```
+
+#### NTreleaseHigh episodes
+
+```Gen
+episode: NTreleaseHigh
+  contained_by: BEH-PRE
+
+  in_context: (Ca2MicroFull AND RRPFull)
+  rf: ( active: 6x ) # High
+
+  hypothesis: NOT (NT empty) # solo in questo caso NT modera!
+    action: [Rrp decrease, Nt increase, ATP decrease]
+    trace: None
+```
+
+#### NTreleaseMedium episodes
+
+```Gen
+episode: NTreleaseMedium
+  contained_by: BEH-PRE
+
+  in_context: (Ca2MicroFull AND RRPMedium) OR (Ca2MicroMedium AND RRPFull)
+  rf: ( active: 6x ) # Medium
+
+  hypothesis: (NT empty) OR NOT (NT empty) # In tutti i casi
+    action: [Rrp decrease, Nt increase, ATP decrease]
+    trace: None
+```
+
+#### NTreleaseLow episodes
+
+```Gen
+episode: NTreleaseLow
+  contained_by: BEH-PRE
+
+  in_context: (Ca2MicroMedium AND RRPMedium)
+  rf: ( active: 6x ) # Low
+
+  hypothesis: (NT empty) OR NOT (NT empty) # In tutti i casi
+    action: [Rrp decrease, Nt increase, ATP decrease]
+    trace: None
+```
+
+## BEH-PRE-VGCC: Container
+
+**Voltage-Controlled Gated Channels**:
+Qui per ora non gestiamo l'evoluzione della depolarizzazione. Alla scomparsa dell'AP, i VGCC smettono di funzionare.
+
+```Gen
+container: BEH-PRE-VGCC
+
+  tub_intricated:
+   - Ca2+ ( contained_by: BEH-PRE )
+
+ context_intricated:
+    - AP ( contained_by: BEH-SOMA )
+```